SoftwareApplication
identifier: 10.4121/16434795
name: Software related to "Impact of optical aberrations on axial position determination by photometry"
description: Software related to "Impact of optical aberrations on axial position determination by photometry"
description: Software distributionThis software is distributed as accompanying software for the article Impact of optical aberrations on axial position determination by photometry by Rasmus Ø. Thorsen, Christiaan N. Hulleman, Mathias Hammer, David Grünwald, Sjoerd Stallinga and Bernd Rieger. This distribution contains Matlab software to run the algorithms described in the article. MatlabThe provided scripts use Matlab (https://mathworks.com) and have been tested working in version R2017b. The scripts uses help functions from the DIPimage Toolbox and must be installed to make full use of the functions. DIPimage is a freely available image processing toolbox for Matlab (http://www.diplib.org). In the directory matlabfun all relevant Matlab functions are included. There are three examples showcasing different computations: example1.m, shows the estimated photon count for a measured bead by a fully-fledged vectorial or simplified Gaussian PSF model compared to TRABI, example2.m, computes a vectorial through-focus PSF and the corresponding photometric ratio (Gaussian fit over TRABI value) as a function of the axial position, example3.m, computes the photometric ratio over six bead measurements. These examples depict part of the data shown in the figure from the article. We hope that these examples are instructive enough to allow the interested user to apply our code. If you have any troubles please to not hesitate to contact us at the email address given below. Data availabilityAdditional data is available for download at: https://doi.org/10.4121/uuid:557b6445-5d40-402a-b214-93d7c6415195. Terms of use Copyright (C) 2018 This program is free software: you can redistribute it and/or modify it under the terms of the GNU General Public License as published by the Free Software Foundation, either version 3 of the License, or (at your option) any later version. This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY; without even the implied warranty of MER…
author: https://orcid.org/0000-0001-6458-4525
author: 0000-0002-0330-913X
author: Person
  given name: Mathias
  family name: Hammer
  name: Mathias Hammer
author: Person
  given name: David
  family name: Grünwald
  name: David Grünwald
author: Person
  given name: Sjoerd
  family name: Stallinga
  name: Sjoerd Stallinga
author: Person
  given name: Bernd
  family name: Rieger
  name: Bernd Rieger
keywords: Photon count estimation
keywords: Vectorial PSF
keywords: PSF fitting
keywords: Photometry
keywords: Axial position
keywords: single-molecule localization microscopy
keywords: Optical aberrations
keywords: TRABI
keywords: Gaussian fitting
license:
  creative work: creativeWork
    name: GPL-3.0-only
reference publication: s41592-018-0227-4

https://orcid.org/0000-0001-6458-4525
type: Person
given name: Rasmus
family name: Thorsen
name: Rasmus Thorsen

0000-0002-0330-913X
type: Person
given name: Christiaan
family name: Hulleman
name: Christiaan Hulleman

s41592-018-0227-4
type: ScholarlyArticle
name: Impact of optical aberrations on axial position determination by photometry
pagination: 989-990
url: https://doi.org/10.1038%2Fs41592-018-0227-4